Independent Variable
Dependent Variable
Method for measuring changes in the D.V.
Control group
Constants
Number of trials
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The temperature of the enzyme catalase is the independent variable.
The rate at which hydrogen peroxide is broken down into water and oxygen gas by catalase is the dependent variable.
The method for measuring changes in the dependent variable is by observing how fast the reaction happens in each test tube.
The test tube with water and liver at room temperature was the control group.
The size of the test tubes, amount of H2O2 used, amount of water used and the time that the water and liver test tubes were boiled and cooled for were the constants.
Only one trial was conducted.
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Test Tube #1
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Water
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Hypothesis:
N/A |
Observations:
N/A |
Interpretations:
N/A |
Test Tube #2
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Water & Liver
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Hypothesis:
There will be no reaction between the water and liver. |
Observations:
Some bubbles were formed. |
Interpretations:
N/A |
Test Tube #3
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Water, Liver, & H2O2
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Hypothesis:
The enzyme catalase in the liver will break down hydrogen peroxide to produce water and oxygen. |
Observations:
Lots of air bubbles were produced. The liver was slowly rising up the test tube. There were bubbles on the liver on top of the test tube. The water became blurry. |
Interpretations:
The reaction happened pretty fast. The enzyme catalase was able to break down H2O2 into water and oxygen. My hypothesis was supported. |
Test Tube #4
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Boiled water and liver & H2O2
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Hypothesis:
The enzyme catalase will be denatured and will not be able to react with H2O2 and break it down. |
Observations:
The water became blurry. Some bubbles were formed. The liver stayed at the bottom. |
Interpretations:
Boiling the enzyme denatured it because the temperature went past the optimum temperature of the enzyme. So, the enzyme did not break down the H2O2. My hypothesis was supported. |
Test Tube #5
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Cooled water and liver & H2O2
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Hypothesis:
The enzyme catalase will not be able to function due to the freezing temperature. |
Observations:
Lots of bubbles were formed and they came out of the test tube. The liver slowly rose to the top and had lots of bubbles on it. |
Interpretations:
The enzyme still functioned even though it was cooled. The temperature of the enzyme must have still been in the range of proper function. My hypothesis was not supported. |